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random hexanucleotide primer kit  (TaKaRa)


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    Structured Review

    TaKaRa random hexanucleotide primer kit
    Random Hexanucleotide Primer Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+hexanucleotide+primer+kit/Random+Primer/pm28509393-218-13-21
    Average 96 stars, based on 6525 article reviews
    random hexanucleotide primer kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Labeling:

    Article Title: NmeA, a novel efflux transporter specific for nucleobases and nucleosides, contributes to metal resistance in Aspergillus nidulans.
    Article Snippet: .. [32P]- dCTP labeled molecules used as nmeA specific probes were prepared using a random hexanucleotide primer kit following the supplier’s instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier’s instructions (Roche Diagnostics, Hellas). .. Labeled [32P]- dCTP (3000 Ci mmol-1) was purchased from the Insitute of Isotops Co. Ltd, Miklos, Hungary.

    Article Title: Oligomerization of the UapA Purine Transporter Is Critical for ER-Exit, Plasma Membrane Localization and Turnover.
    Article Snippet: .. [ 32 P]-dCTP labeled molecules of uapA, argB or pabaA specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier's instructions (Roche Diagnostics, Hellas). ..

    Article Title: The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi
    Article Snippet: .. [ 32 P]-dCTP labeled molecules of gene specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpin S-200 HR columns (Roche Diagnostics, Hellas). .. Labeled [ 32 P]-dCTP (3000 Ci mmol −1 ) was purchased from the Institute of Isotops Co. Ltd, Miklós, Hungary.

    Article Title: Origin, diversification and substrate specificity in the family of NCS1/FUR transporters.
    Article Snippet: 5 Faculty of Biology, University of Athens, Panepistimioupolis, Athens 15784, Greece.. 6 Faculty of Pharmacy, University of Athens, Panepistimioupolis, Athens 15771, 7 Greece.. 8 3 Bioinformatics and Genomics Programme.

    Article Title: Cryptic purine transporters in Aspergillus nidulans reveal the role of specific residues in the evolution of specificity in the NCS1 family.
    Article Snippet: .. [32P]-dCTP labeled molecules used as fur or pantoB specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier's instructions (Roche Diagnostics, Hellas). .. Labeled [32P]-dCTP (3000 Ci mmol-1) was purchased from the Institute of Isotops Co. Ltd, Miklós, Hungary.

    Article Title: The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi
    Article Snippet: .. [32P]-dCTP labeled molecules of gene specific probes were prepared using a random hexanucleotide primer kit following the supplier’s instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on Figure 10 continued and SagA endocytic factors might have roles in both the AP-2 and clathrin endocytic pathways. ..

    Article Title: The Aspergillus nidulans Proline Permease as a Model for Understanding the Factors Determining Substrate Binding and Specificity of Fungal Amino Acid Transporters
    Article Snippet: Plasmid preparation from Escherichia coli strains and DNA bands purified from agarose gels were performed with the Nucleospin Plasmid kit and the Nucleospin ExtractII kit, respectively, according to the manufacturer's instructions (Macherey-Nagel, Lab Supplies Scientific SA, Hellas, Greece). .. DNA probes for Southern analysis ( 54 ) were labeled with [ 32 P]dCTP (3000 Ci/mmol, Institute of Isotopes Co. Ltd., Miklós, Hungary) using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA) and purified on MicroSpin TM S-200 HR columns following the supplier's instructions (Roche Diagnostics). .. Restriction enzymes used were from Takara Bio. table ft1 table-wrap mode=article t1 TABLE 2 caption a4 Primers used in this study Epifluorescence Microscopy Samples were prepared as previously described ( 55 , 56 ).

    Purification:

    Article Title: NmeA, a novel efflux transporter specific for nucleobases and nucleosides, contributes to metal resistance in Aspergillus nidulans.
    Article Snippet: .. [32P]- dCTP labeled molecules used as nmeA specific probes were prepared using a random hexanucleotide primer kit following the supplier’s instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier’s instructions (Roche Diagnostics, Hellas). .. Labeled [32P]- dCTP (3000 Ci mmol-1) was purchased from the Insitute of Isotops Co. Ltd, Miklos, Hungary.

    Article Title: Oligomerization of the UapA Purine Transporter Is Critical for ER-Exit, Plasma Membrane Localization and Turnover.
    Article Snippet: .. [ 32 P]-dCTP labeled molecules of uapA, argB or pabaA specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier's instructions (Roche Diagnostics, Hellas). ..

    Article Title: The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi
    Article Snippet: .. [ 32 P]-dCTP labeled molecules of gene specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpin S-200 HR columns (Roche Diagnostics, Hellas). .. Labeled [ 32 P]-dCTP (3000 Ci mmol −1 ) was purchased from the Institute of Isotops Co. Ltd, Miklós, Hungary.

    Article Title: Origin, diversification and substrate specificity in the family of NCS1/FUR transporters.
    Article Snippet: 5 Faculty of Biology, University of Athens, Panepistimioupolis, Athens 15784, Greece.. 6 Faculty of Pharmacy, University of Athens, Panepistimioupolis, Athens 15771, 7 Greece.. 8 3 Bioinformatics and Genomics Programme.

    Article Title: Cryptic purine transporters in Aspergillus nidulans reveal the role of specific residues in the evolution of specificity in the NCS1 family.
    Article Snippet: .. [32P]-dCTP labeled molecules used as fur or pantoB specific probes were prepared using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on MicroSpinTM S-200 HR columns, following the supplier's instructions (Roche Diagnostics, Hellas). .. Labeled [32P]-dCTP (3000 Ci mmol-1) was purchased from the Institute of Isotops Co. Ltd, Miklós, Hungary.

    Article Title: BsdA(Bsd2) -dependent vacuolar turnover of a misfolded version of the UapA transporter along the secretory pathway: prominent role of selective autophagy.
    Article Snippet: Transmembrane proteins translocate cotranslationally in the endoplasmic reticulum (ER) membrane and traffic as vesicular cargoes, via the Golgi, in their final membrane destination.. Misfolding in the ER leads to protein degradation basically through the ERAD/proteasome system.. Here, we use a mutant version of the purine transporter UapA (DR481) to show that specific misfolded versions of plasma membrane cargoes undergo vacuolar turnover prior to localization in the plasma membrane.

    Article Title: The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi
    Article Snippet: .. [32P]-dCTP labeled molecules of gene specific probes were prepared using a random hexanucleotide primer kit following the supplier’s instructions (Takara Bio, Lab Supplies Scientific SA, Hellas) and purified on Figure 10 continued and SagA endocytic factors might have roles in both the AP-2 and clathrin endocytic pathways. ..

    Article Title: The Aspergillus nidulans Proline Permease as a Model for Understanding the Factors Determining Substrate Binding and Specificity of Fungal Amino Acid Transporters
    Article Snippet: Plasmid preparation from Escherichia coli strains and DNA bands purified from agarose gels were performed with the Nucleospin Plasmid kit and the Nucleospin ExtractII kit, respectively, according to the manufacturer's instructions (Macherey-Nagel, Lab Supplies Scientific SA, Hellas, Greece). .. DNA probes for Southern analysis ( 54 ) were labeled with [ 32 P]dCTP (3000 Ci/mmol, Institute of Isotopes Co. Ltd., Miklós, Hungary) using a random hexanucleotide primer kit following the supplier's instructions (Takara Bio, Lab Supplies Scientific SA) and purified on MicroSpin TM S-200 HR columns following the supplier's instructions (Roche Diagnostics). .. Restriction enzymes used were from Takara Bio. table ft1 table-wrap mode=article t1 TABLE 2 caption a4 Primers used in this study Epifluorescence Microscopy Samples were prepared as previously described ( 55 , 56 ).



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